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KMID : 1036720150480060459
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Journal of Nutrition and Health
2015 Volume.48 No. 6 p.459 ~ p.467
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Comparing the anti-inflammatory effect of nanoencapsulated lycopene and lycopene on RAW 264.7 macrophage cell line
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서은영:Seo Eun-Youn g
김명환:Kim Myung-Hwan/김우경:Kim Woo-Kyung/장문정:Chang Moon-Jeong
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Abstract
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Purpose: We developed a method to load lycopene into maltodextrin and cyclodextrin in an attempt to overcome the poor bioavailability and improve the anti-inflammatory effect of this polyphenol.
Methods: Nanosized lycopenes were encapsulated into biodegradable amphiphillic cyclodextrin and maltodextrin molecules prepared using a high pressure homogenizer at 15,000~25,000 psi. Cell damage was induced by lipopolysaccharides (LPS) in a mouse macrophage cell line, RAW 264.7. The cells were subjected to various doses of free lycopene (FL) and nanoencapsulated lycopene (NEL). RT-PCR was used to quantify the tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), IL-6, inducible nitric oxide synthase (iNOS), and cyclooxigenase-2 (COX-2) mRNA levels, while ELISA was used to determine the protein levels of TNF-α, IL-1β, and IL-6.
Results: NEL significantly reduced the mRNA expression of IL-6 and IL-1β at the highest dose, while not in cells treated with FL. In addition, NEL treatment caused a significant reduction in IL-6 and TNF-α protein levels, compared to cells treated with a similar dose of FL. In addition, mRNA expression of iNOS and COX-2 enzyme in the activated macrophages
was more efficiently suppressed by NEL than by FL.
Conclusion: Overall, our results suggest that lycopene is a potential inflammation reducing agent and nanoencapsulation of lycopene can further improve its anti-inflammatory effect during tissue-damaging inflammatory conditions.
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KEYWORD
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lycopene, nanoencapsulated lycopene, anti-inflammation, RAW 264.7 cells
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